GENERAL HISTOLOGY

Detailed explanation-1: -Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm.

Detailed explanation-2: -IRIS DIAPHRAGM CONTROL-A lever (or rotating disk) that adjusts the amount of light illuminating the slide. Use just enough light to illuminate the object on the slide and give good contrast.

Detailed explanation-3: -The field diaphragm in the base of the microscope controls only the width of the bundle of light rays reaching the condenser. This variable aperture does not affect the optical resolution, numerical aperture, or the intensity of illumination.

Detailed explanation-4: -To adjust the light intensity and view the specimen at higher magnification, one can adjust the brightness by rotating the light intensity knob. One can also adjust the condenser by bringing it close or far with the help of a condenser knob and also by opening and closing the iris diaphragm.

Detailed explanation-5: -Condenser Aperture Diaphragm Operation The opening and closing of this iris diaphragm controls the angle of illuminating rays (and thus the aperture) which pass through the condenser, through the specimen and then into the objective.