GENERAL HISTOLOGY

Detailed explanation-1: -On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing. If nothing appears, reduce the light and repeat step 4. Once in focus on low power, center the object of interest by moving the slide.

Detailed explanation-2: -Coarse Adjustment Knob-The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus. The gearing mechanism of the adjustment produces a large vertical movement of the stage with only a partial revolution of the knob.

Detailed explanation-3: -At a high power objective, the focus will change rapidly as the objective lens is adjusted, so only the fine focus knob should be used in order to prevent overshooting the correct focal plane.

Detailed explanation-4: -This is the knob on the side of the microscope that moves the objective lens up and down. It is used in conjunction with the fine focus. A focusing system with both the coarse and fine focusing knobs mounted on the same axis. The coarse focus is typically the larger, outside knob and vice versa.