GENERAL HISTOLOGY

Detailed explanation-1: -A scanning electron microscope (SEM) projects and scans a focused stream of electrons over a surface to create an image. The electrons in the beam interact with the sample, thereby producing various signals that can be used to obtain information about the surface’s topography and composition.

Detailed explanation-2: -Scanning electron microscopy (SEM) is normally used for imaging the surface of cells, tissues and whole multicellular organisms. SEM images of surfaces appear to be three-dimensional (3D) but there is no measurable depth information in the image.

Detailed explanation-3: -The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950’s, scanning electron microscopes have developed new areas of study in the medical and physical science communities. The SEM has allowed researchers to examine a much bigger variety of specimens.

Detailed explanation-4: -An electron microscope is defined as the type of microscope in which the source of illumination is the beam of accelerated electrons. It is a special type of microscope with a high resolution of images as the images can be magnified in nanometers.

Detailed explanation-5: -The 3D SEM software identifies homologous points on the images which belong to the same point on the sample. In this way the true three dimensional coordinates of all pixels within the image are calculated to generate a 3D model of the sample.