GENERAL HISTOLOGY

Detailed explanation-1: -Iris or diaphragm is the rotating disc found under the stage. Its main role is to control the amount of light passing through the specimen. It is located below the microscope stage and above the condenser lens. An Abbe condenser with an iris diaphragm is often found in most high-quality microscopes.

Detailed explanation-2: -Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen.

Detailed explanation-3: -Iris Diaphragm is the part of the microscope that is responsible for controlling how much light reaches the ocular lens. The ocular lens is the part of the microscope through which the specimens are observed. It is also known as the eyepiece.

Detailed explanation-4: -The field diaphragm in the base of the microscope controls only the width of the bundle of light rays reaching the condenser. This variable aperture does not affect the optical resolution, numerical aperture, or the intensity of illumination.