GENERAL HISTOLOGY

Detailed explanation-1: -Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. This method will help prevent air bubbles from being trapped under the cover slip.

Detailed explanation-2: -A small square or circle of thin glass called a coverslip is placed over the specimen. It prevents the slide from drying out when it’s being examined.

Detailed explanation-3: -The main function of the cover slip is to keep solid specimens pressed flat, and liquid samples shaped into a flat layer of even thickness. This is necessary because high-resolution microscopes have a very narrow region within which they focus.

Detailed explanation-4: -The specimen will be placed on the slide, usually with a drop of stain, such as iodine solution or methylene blue, to make the transparent cells visible. A coverslip, which is a thin piece of glass, again to allow light to pass through, will be placed on top of the specimen.

Detailed explanation-5: -A mounted needle can be used to hold the specimen in place as the coverslip is lowered. It is important that no air bubbles are trapped underneath. Most cells are colourless. Stains are used to add contrast.