GENERAL HISTOLOGY

Detailed explanation-1: -You can do this vertically or horizontally. Using your other hand, angle the bottom edge of the coverslip to a 45-degree angle at the bottom of the slide. Slowly and gently lower the coverslip onto the slide so it covers the sample.

Detailed explanation-2: -Why is it important to apply a cover slip at a 45 degree angle when making a wet mount? Applying the cover slip at an angle (instead of dropping it down flat on the specimen) pushes the air to the side and therefore minimizes the risk of air bubbles.

Detailed explanation-3: -Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. This method will help prevent air bubbles from being trapped under the cover slip.

Detailed explanation-4: -Do not drop the cover slip straight down onto the drop. This will have a tendency to trap air under the cover slip, resulting in air bubbles that may interfere with specimen viewing. Rather, drop the cover slip from about a 45 degree angle push out most of the air as the drop is spread out.

Detailed explanation-5: -It has to be placed carefully onto the specimen to prevent trapping air bubbles beneath it, which would spoil the image you see of the specimen. The technique is to put the coverslip at an angle of about 45 degrees on the edge of the solution on the slide.