GENERAL HISTOLOGY

INTRODUCTION

MICROSCOPE PARTS MAGNIFICATION RESOLUTION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
When preparing a wet mount slide of onion cells, a student put a drop of Lugol’s iodine on the slide. This iodine was added to:
A
prevent air bubbles
B
make cell structures more visible
C
increase the magnification
D
increase the rate of photosynthesis in cells
Explanation: 

Detailed explanation-1: -When preparing a wet-mount slide of onion cells, a student put a drop of LUGOL’s IODINE STAIN on the slide. Lugol’s iodine stain was added to me cell structures more VISIBLE. While viewing a specimen under high power on a compound light microscope, a student noticed that the specimen was OUT OF FOCUS.

Detailed explanation-2: -The iodine acts as a stain to make the structures in the cell easier to see. Carefully lower a cover slip over the onion skin. Try to avoid trapping air bubbles. Place the slide on the microscope stage.

Detailed explanation-3: -Preparing A Wet-Mount Onion Skin Slide Cut the onion into sections. Take a piece from on of the sections and peel off a small, thin piece of the onion epidermis, or skin. Using tweezers, place the onion skin onto the drop of water on the slide. Now, drop a small amount of iodine onto the onion skin.

Detailed explanation-4: -Remove a fleshy leaf from the onion bulb. Strip a thin layer with forceps. Place a piece of it in a drop of water on a clean side. With a dropper, add a drop of iodine onto it. Gently cover with a cover slip. Use a piece of filter paper to remove excess liquid from the slide. More items

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