GENERAL HISTOLOGY

Detailed explanation-1: -On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing.

Detailed explanation-2: -Which Microscope Objective Should I Start With? Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample.

Detailed explanation-3: -When using a microscope, you should always start with the lowest power objective when first viewing a specimen. Typically light microscopes have 4x, 10, 40x, and 100x objectives, therefore, you should start with the 4x objective.

Detailed explanation-4: -Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

Detailed explanation-5: -How to Focus Your Microscope: The proper way to focus a microscope is to start with the lowest power objective lens first and while looking from the side, crank the lens down as close to the specimen as possible without touching it. Now, look through the eyepiece lens and focus upward only until the image is sharp.