GENERAL HISTOLOGY

Detailed explanation-1: -By placing immersion oil between the glass slide and the oil immersion lens (100X), the light rays at the highest magnification can be retained. Immersion oil has the same refractive index as glass so the oil becomes part of the optics of the microscope.

Detailed explanation-2: -Before using immersion oil, make sure that your 100x objective lens is made for use with immersion oil. Begin by focusing your sample using the 40x objective lens. Rotate the objective lens part way between the 40x and 100x lens so you can reach the cover slip on your slide.

Detailed explanation-3: -Oil Immersion Objective Lens (100x) The oil immersion objective lens provides the most powerful magnification, with a whopping magnification total of 1000x when combined with a 10x eyepiece.

Detailed explanation-4: -100x lens and Oil Immersion This requires a special 100x objective. The 100x lens is immersed in a drop of oil placed on the slide in order to eliminate any air gaps and lossof light due to refraction (bending of the light) as the light passes from glass (slide) → air → glass (objective lens).

Detailed explanation-5: -The microscope immersion oil decreases the light refraction, allowing more light to pass through your specimen to the objectives lens. Therefore, the microscope immersion oil increases the resolution and improve the image quality.