GENERAL HISTOLOGY

Detailed explanation-1: -Which Microscope Objective Should I Start With? Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample.

Detailed explanation-2: -When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you’re looking for.

Detailed explanation-3: -Scanning Objective Lens (4x) A scanning objective lens provides the lowest magnification power of all objective lenses. 4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of 40x.

Detailed explanation-4: -Answer and Explanation: You should use the 5x objective for your initial examination. This is the lowest power objective that is used for scanning the slide. By using the scanning objective (which has the largest field of view), you are able to quickly get a sense of the composition of the slide.

Detailed explanation-5: -Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing.