GENERAL HISTOLOGY

Detailed explanation-1: -The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power. Therefore, it is best to find an object on low power, center it, and then switch to the next higher power and repeat.

Detailed explanation-2: -Which Microscope Objective Should I Start With? Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample.

Detailed explanation-3: -When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see. This makes it easier to find what you’re looking for.

Detailed explanation-4: -Low Power Objective (10x) The low power objective lens has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples.

Detailed explanation-5: -The low power objective on many microscopes is 10x, other common low magnification objectives are 4x, 6.3x, and 16x. Low power objectives have the largest working distance, and it is good to get in the habit of selecting the low power objective before placing the specimen on the stage.