GENERAL HISTOLOGY

Detailed explanation-1: -Some cell organelles cannot be seen with light microscopes as they cannot achieve a high magnification. Organelles which can be seen under light microscope are nucleus, cytoplasm, cell membrane, chloroplasts and cell wall. Mitochondria are also visible under light microscope but detailed study is not possible.

Detailed explanation-2: -Since electron microscopy cannot be performed on living tissue (the electron beams completely destroy live cells) [59], light microscopy is the primary tool for visualizing mitochondrial function within cells.

Detailed explanation-3: -The mitochondrial outer membrane contains numerous transporters and channels, most of which are so abundant that their distributions cannot be resolved with diffraction-limited microscopy.

Detailed explanation-4: -With light microscopy, one cannot visualize directly structures such as cell membranes, ribosomes, filaments, and small granules and vesicles.

Detailed explanation-5: -Lysosomes/Endosome. Again, individual endosomes and lysosomes are not visible using regular light microscopy.