GENERAL HISTOLOGY

Detailed explanation-1: -Light rays emanating from the eyepiece intersect at the exit pupil or eyepoint, often referred to as the Ramsden disk, where the pupil of the microscopists eye should be placed in order to see the entire field of view (usually 8–10 mm from the eye lens).

Detailed explanation-2: -The circle you can see through the eyepiece is called the field of view. Note: one of the oculars may have a pointer used to identify a specific area on the slide.

Detailed explanation-3: -The eyepiece produces a virtual image requiring an additional lens. The microscopist’s eye lens serves this purpose (figure 6.5). The virtual image “exists” outside the microscope at the location of the specimen. The lens in the microscopist’s eye converts this image into a real image on the retina.

Detailed explanation-4: -Transmitted light usually gets to the specimen from below the stage by means of an illuminator. It’s the most frequently used lighting for compound, high-power microscopy. The simplest illuminator is a pivoted mirror to beam external light to the microscope.

Detailed explanation-5: -Look through the eyepiece and move the Adjustment knob so that the lens lifts away from the slide. The image should come into focus. Be careful not to drop the lens into the face of the slide, as this may cause damage to the lens. The condenser can be adjusted to increase or decrease light intensity.