GENERAL HISTOLOGY

Detailed explanation-1: -CAUTION: NEVER, EVER, use the coarse adjustment knob with the high power objective. This could break the lens. Multiply the power of the ocular lens, by the power of the objective lens. 2.

Detailed explanation-2: -The coarse adjustment knob is used ONLY with the low power (4X, 10X) objectives. When focusing under the 40X or 100X objective, ONLY use the fine adjustment, never the coarse adjustment. Refer to each of the following procedures every time the microscope is used.

Detailed explanation-3: -Use the coarse adjustment to bring the object into focus. Always turn the coarse focus knob so the objective lens moves away from the stage, so that you will not break the slide or damage the lens. Never use the coarse adjustment to focus closer to the object while looking through the eyepiece.

Detailed explanation-4: -If you focus the high power objectives with the coarse focus knob, then you risk slamming the objective into the slide, risking the damage of both. Some high power objectives are spring-loaded, with a front-part that retracts when touched by the slide. This is a protective mechanism.

Detailed explanation-5: -4. COARSE ADJUSTMENT KNOB-A rapid control which allows for quick focusing by moving the objective lens or stage up and down. It is used for initial focusing.