GENERAL HISTOLOGY

Detailed explanation-1: -Safranine is used to stain onion peel cells. This makes them easily observable under a microscope.

Detailed explanation-2: -Since there are starch molecules in onion epidermal cells, the addition of iodine solution to a specimen of these cells allows the iodine to infiltrate the epidermal cells and bind to the starch molecules present. This gives color to the epidermal cells, making them more readily visible and observable.

Detailed explanation-3: -Iodine– to stain the cells so it can be seen under the microscope. Not all cells can be stained with iodine, but onion cells take it up nicely so that there is contrast under the microscope. Onion– Peeled to one layer. If it’s any thicker, you won’t be able to see through it under the microscope.

Detailed explanation-4: -A wet mount of an onion membrane is used to represent plant cells and is stained with an iodine stain that reacts with any starch present to produce a blue/black colour.

Detailed explanation-5: -Iodine is used in staining cells of an onion peel on the slide due to following reason. *Onion bulb is made up of scaly leaves which store starch granules in their cytoplasm of the cells. *Iodine binds to starch in the granules and develops blue black colour.