GENERAL HISTOLOGY

Detailed explanation-1: -The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.

Detailed explanation-2: -Plants and animals specimens are stained with dyes before observing them through a microscope because cells appear transparent under the microscope and to make the internal organelles and other structures clearly visible, different stains or dyes are used for different parts of the cells.

Detailed explanation-3: -Staining is used to highlight important features of the tissue as well as to enhance the tissue contrast. Hematoxylin is a basic dye that is commonly used in this process and stains the nuclei giving it a bluish color while eosin (another stain dye used in histology) stains the cell’s nucleus giving it a pinkish stain.

Detailed explanation-4: -Some stains can penetrate cell walls and highlight cell components, and this can help scientists visualize metabolic processes. Stains also help distinguish between live cells and dead ones. Moreover, staining allows scientists count the number of cells of a particular type within a certain biomass.

Detailed explanation-5: -By staining onion peel, it will make the cell wall more transparent in an onion cell. It adds contrast when seen through a microscope. Methylene Blue is a blue dye that can be used to colour blood, bacteria, acidic or protein-rich cell structures such as the nucleus, ribosomes, and endoplasmic reticulum.