GENERAL HISTOLOGY

Detailed explanation-1: -The scanning electron microscope (SEM) lets us see the surface of three-dimensional objects in high resolution. It works by scanning the surface of an object with a focused beam of electrons and detecting electrons that are reflected from and knocked off the sample surface.

Detailed explanation-2: -Scanning electron microscopy (SEM) is normally used for imaging the surface of cells, tissues and whole multicellular organisms. SEM images of surfaces appear to be three-dimensional (3D) but there is no measurable depth information in the image.

Detailed explanation-3: -The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950’s, scanning electron microscopes have developed new areas of study in the medical and physical science communities. The SEM has allowed researchers to examine a much bigger variety of specimens.

Detailed explanation-4: -SEMs provide a 3D image of the surface of the sample, whereas TEM images are 2D projections of the sample, which in some cases makes the interpretation of the results more difficult for the operator.

Detailed explanation-5: -In 3D electron microscopy, multiple sets of stereoscopic images taken from a range of viewing angles are collated and digitally constructed into a three-dimensional dataset. It is similar to computed tomography (CT) where samples are imaged as a series of two-dimensional cross-sections.