GENERAL HISTOLOGY

Detailed explanation-1: -Shorter wavelengths are able to resolve smaller objects; thus, an electron microscope has a much higher resolution than a light microscope, since it uses an electron beam with a very short wavelength, as opposed to the long-wavelength visible light used by a light microscope.

Detailed explanation-2: -Resolution is also related to the wavelength of light which is used to image a specimen; light of shorter wavelengths are capable of resolving greater detail than longer wavelengths.

Detailed explanation-3: -Different types of microscope have different resolving powers. Light microscopes let us distinguish objects as small as a bacterium. Electron microscopes have much higher resolving power – the most powerful allow us to distinguish individual atoms.

Detailed explanation-4: -The greatest resolving power in optical microscopy is realized with near-ultraviolet light, the shortest effective imaging wavelength. Near-ultraviolet light is followed by blue, then green, and finally red light in the ability to resolve specimen detail.

Detailed explanation-5: -The theoretical resolution of electron microscopes is 0.23 nm for an accelerating voltage 100keV ( = 0.003701nm) and 0.12 nm for 300keV ( = 0.001969 nm), and is mainly limited by the quality of electron optics.