GENERAL HISTOLOGY

Detailed explanation-1: -In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent.

Detailed explanation-2: -Wet-mount Slides A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Advantages-This type of slide preparation allows you to view microscopic living things without them drying out.

Detailed explanation-3: -The cover slip protects the ocular lens from damage by acting as a barrier between it and the specimen. The slide itself also helps to keep the stage, or the part of the microscope below the lens, clean.

Detailed explanation-4: -The slide is first prepared and then placed on the microscope stage. The mirror reflects the light onto the stage through the condenser. The lens through which an individual sees the slide is called the eyepiece. There are objectives of different magnification power such as 10x, 40x and 100x.

Detailed explanation-5: -Place one side of a coverslip at an angle so that its edge touches the slide and the outer edge of the drop. Slowly lower the coverslip, avoiding air bubbles. Most problems with air bubbles come from not applying the coverslip at an angle, not touching the liquid drop, or from using a viscous (thick) liquid.