GENERAL HISTOLOGY

Detailed explanation-1: -The amount of light transmitted to your eye is greatest at the low power. When you switch to a higher power, light (and therefore resolving power, or the ability to distinguish two nearby objects as separate) is reduced.

Detailed explanation-2: -The resolving power of an objective lens is measured by its ability to differentiate two lines or points in an object. The greater the resolving power, the smaller the minimum distance between two lines or points that can still be distinguished.

Detailed explanation-3: -The greatest resolving power in optical microscopy is realized with near-ultraviolet light, the shortest effective imaging wavelength. Near-ultraviolet light is followed by blue, then green, and finally red light in the ability to resolve specimen detail.

Detailed explanation-4: -The best resolution for an optical microscope is about 0.2 microns = 200 nm. The good news is, there’s a difference between resolution and “ability to locate the position". If you have one tiny and isolated fluorescent object, you can often locate the position of that object to better than your resolution.

Detailed explanation-5: -The resolving power of the microscope increases with the decrease in wavelength of light and an increase in the numerical aperture.