ABDOMINAL
THALASSEMIA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The principle for hemoglobin electrophoresis is based on which of the following?
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Net charge of the hemoglobin molecule
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Amount of hemoglobin present
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Structure of heme portion
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Temperature
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Explanation:
Detailed explanation-1: -Electrophoresis is a separation technique based on the mobility of ions in an electric field. It is the classical method of identifying and quantifying the haemoglobin proteins. The haemoglobin molecules (HbA, HbA2, HbF and variants) in solution are electrically charged at any given pH.
Detailed explanation-2: -In alkaline electrophoresis, hemoglobin molecules assume a negative charge and migrate toward the anode (positive pole).
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