HUMAN NUTRITION

NUTRITION

CARBOHYDRATES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Test used to detect protein in a sample:
A
Biuret test
B
Iodine test
C
Benedict’s test
D
Brown paper bag test
Explanation: 

Detailed explanation-1: -A small amount of this aqueous solution must be treated with 1% sodium hydroxide or potassium hydroxide. To this mixture, a few drops of CuSO4 (aq) must be added. If the solution turns purple upon the addition of copper(II) sulphate, the presence of protein in the analyte is confirmed.

Detailed explanation-2: -The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.

Detailed explanation-3: -all peptides and protein give the test positive. Histidine is the only amino acid that give biuret test positive.

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