NUTRITION
CARBOHYDRATES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The following are the sugars that formed brick red precipitate during Barfoed test, EXCEPT:
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Sucrose
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Lactose
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Glucose
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Fructose
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Explanation:
Detailed explanation-1: -Barfoed’s test is also based on the reducing ability of sugar. However, sucrose also gives this test positive as it undergoes hydrolysis in the presence of an acid. Monosaccharides give early positive test while the disaccharides give late positive.
Detailed explanation-2: -Barfoed’s test is used to detect the presence of monosaccharide (reducing) sugars in solution. Barfoed’s reagent, a mixture of ethanoic (acetic) acid and copper(II) acetate, is combined with the test solution and boiled. A red copper(II) oxide precipitate is formed will indicates the presence of reducing sugar.
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