NUTRITION
CARBOHYDRATES
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Barfoed Test
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Osazone formation
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Seliwanoff’s test
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Bial’s Orcinol test
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Detailed explanation-1: -Seliwanoff’s test is used to differentiate between sugars that have a ketone group (ketose) and sugars that have an aldehyde group (aldoses). This test is a timed color reaction specific to ketohexoses. To detect the presence of ketohexoses in a given sample.
Detailed explanation-2: -Seliwanoff’s Test uses 6 M HCl as dehydrating agent and resorcinol as condensation reagent. The test reagent dehydrates ketohexoses to form 5-hydroxymethylfurfural. 5-hydroxymethylfurfural further condenses with resorcinol present in the test reagent to produce a cherry red product within two minutes.
Detailed explanation-3: -Results of Seliwanoff’s test Negative Test: If you find that no red color or even a slight pink tint is visible the result is negative and indicates that Aldose sugar (Glucose) is present in the solution.