HUMAN NUTRITION

NUTRITION

LIPIDS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
If Sudan III is red it is a ____
A
negative test for a lipid
B
negative test for a simple sugar
C
indicates the presence of a lipid
D
detects a protein
Explanation: 

Detailed explanation-1: -Hypothesis : If the lipids are mixed with sudan III, then red color will cause the lipids to be more visible then other substances.

Detailed explanation-2: -The Reaction: Sudan III reacts with the lipids or triglycerides to stain red in colour.

Detailed explanation-3: -RESULTS: Denim-blue = negative. Lavender = positive. Water plus Biuret’s reagent is a negative control for the protein test. It demonstrates a negative test result (no protein present).

Detailed explanation-4: -Positive result: Lipids are soluble in a non-polar solvent, i.e. chloroform and partially soluble in ethanol which can solubilize upon heating. Negative result: Lipids are insoluble in a polar solvent, i.e. water.

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