HUMAN NUTRITION

Detailed explanation-1: -The Liebermann–Burchard or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive. Lieberman–Burchard is a reagent used in a colourimetric test to detect cholesterol, which gives a deep green colour.

Detailed explanation-2: -In the Liebermann-Burchard (LB) colorimetric assay, treatment of cholesterol with sulfuric acid, acetic anhydride, and acetic acid elicits a blue color.

Detailed explanation-3: -Conclusions It has been demonstrated that the Liebermann–Burchard reaction via colorimetric method could be used in successful analysis of cholesterol in dairy products.

Detailed explanation-4: -Liebermann – Burchard test is a chemical estimation of cholesterol. The cholesterol is react as a typical alcohol with a strong concentrated acids to give colored substances. In this test acetic anhydride are used as solvent and dehydrating agents, and the sulfuric acid is used as dehydrating and oxidizing agent.