NUTRITION
CARBOHYDRATES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The following are materials used in the Isolation of Liver Glycogen experiment, EXCEPT:
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95% Ethanol
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Sand
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5% TCA
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Table sugar
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Explanation:
Detailed explanation-1: -Barfoed’s test reaction is based on the reduction of cupric acetate by reducing monosaccharides and reducing disaccharides. Reduction of cupric acetate produces cuprous oxide which gives a brick red precipitate.
Detailed explanation-2: -a) Fehling’s Test: Fehling’s Solution (deep blue colored) is used to determine the presence of reducing sugars and aldehydes. Perform this test with fructose, glucose, maltose and sucrose.
Detailed explanation-3: -Positive Bial’s test: formation of blue color ( eg. Ribose sugar) Negative Bial’s test: formation of any other color indicates negative test. Hexose sugar ( glucose, fructose) generally gives green, red or brown color product.
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