BIOCHEMISTRY
ENZYMES AND METABOLISM
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Choose the incorrect statement on Km.
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The unit for length is cm. The unit for Km is substrate concentration
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Km is the value of [S] when the velocity is half Vmax
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Km can be obtained directly from Lineweaver-Burke plot
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Km is a Michaelis-Menten constant
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Explanation:
Detailed explanation-1: -Lineweaver-Burk plot. This equation can be compared with the equation for a straight line: y = mx + b, where m is the slope and b is the y-intercept. In the Lineweaver-Burk equation, Km/Vmax is the slope (or m) and 1/Vmax is the y-intercept (or b).
Detailed explanation-2: -Vmax and Km can be determined from linear regression analysis of a plot of 1/Vo vs. 1/[S]o, a so-called Lineweaver-Burk plot. Figure one A Lineweaver-Burk plot of enzyme kinetic data. In a Lineweaver-Burk plot the inverse of the x and y-intercepts represent the kinetics constants Km and Vmax respectively.
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