AP BIOLOGY

LABORATORY REVIEW

ENZYME CATALYSIS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
With the addition of the enzyme alcohol dehydrogenase, the rate of reduction of acetaldehyde to ethanol increases as the concentration of acetaldehyde is increased. Eventually, the rate of the reaction reaches a maximum, at which point further increases in the concentration of acetaldehyde have no effect. Why?
A
All the alcohol dehydrogenase molecules are bound to acetaldehyde molecules.
B
At high concentrations of acetaldehyde, the activation energy of the reaction increases.
C
At high concentrations of acetaldehyde, the activation energy of the reaction decreases.
D
The enzyme is no longer specific for acetaldehyde.
E
At high concentrations of acetaldehyde, the change in free energy of the reaction decreases.
Explanation: 

Detailed explanation-1: -In the presence of alcohol dehydrogenase, the conversion of acetaldehyde to ethanol increases as the concentration of acetaldehyde is increased. Eventually, the rate of the reaction reaches a maximum, after which point further increases in the concentration of acetaldehyde have no effect.

Detailed explanation-2: -Acetaldehyde Dehydrogenase (CoA-Acetylating) It was then discovered in the strictly anaerobic bacterium Clostridium kluyveri [135]. The enzyme is responsible for the conversion of acetyl-coenzyme A (acetyl-CoA) to acetaldehyde that is eventually converted to ethanol.

Detailed explanation-3: -The enzyme alcohol dehydrogenase (ADH) metabolizes the ethanol (that’s the type of alcohol in alcohol) into toxic acetaldehyde. From there the liver enzyme aldehyde dehydrogenase (ALDH) metabolizes acetaldehyde into acetate, a less toxic compound that breaks down into water and carbon dioxide.

Detailed explanation-4: -The NAD Cofactor Alcohol dehydrogenase uses two molecular tools to convert ethanol to acetaldehyde. The first is a zinc atom which is used to hold and position the alcohol group on ethanol. The second is a large NAD cofactor, which actually performs the reaction.

Detailed explanation-5: -Most of the ethanol in the body is broken down in the liver by an enzyme called alcohol dehydrogenase (ADH), which transforms ethanol into a toxic compound called acetaldehyde (CH3CHO), a known carcinogen.

There is 1 question to complete.