THE MOLECULAR BASIS OF INHERITANCE
THE HUMAN GENOME
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Bacterial Artificial Chromosome
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Baked Artificial Cake
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Biotechnology And Cry genes
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Detailed explanation-1: -For the amplification of separated fragments in the process, these are attached to the vectors like BAC(bacterial artificial chromosome and YAC(yeast artificial chromosome) and cloned inside the host bacteria or yeast. Hence, the correct answer is BAC and YAC.
Detailed explanation-2: -YAC vectors are DNA constructs that are used for cloning DNA in yeasts. BAC vectors are DNA constructs that are used for cloning DNA in bacteria. Gene insert. A gene sequence of 100-1000 kbp can be inserted in these vectors. A gene sequence of 150-350 kbp can be inserted into the BAC vectors.
Detailed explanation-3: -The Bac-to-Bac baculovirus expression system enables the efficient production of recombinant baculovirus for expression testing in insect cells. The system relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
Detailed explanation-4: -YAC stands for Yeast Artificial Chromosome and BAC stands for Bacterial artificial chromosome. YAC are derived from DNA of yeast S. cerevisiae by the process of genetic engineering. They are then ligated into the bacterial plasmid.
Detailed explanation-5: -The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes.