AP BIOLOGY

Detailed explanation-1: -Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.

Detailed explanation-2: -The only way to increase the reaction rate once the saturation point is reached is to increase the amount of enzymes.

Detailed explanation-3: -When an enzyme binds its substrate, it forms an enzyme-substrate complex. This complex lowers the activation energy of the reaction and promotes its rapid progression by providing certain ions or chemical groups that actually form covalent bonds with molecules as a necessary step of the reaction process.

Detailed explanation-4: -Increasing enzyme concentration increases the activity because there are more active sites present to attach to the substrates present.

Detailed explanation-5: -As the substrate concentration increases, the enzyme reaction increases until all of the active sites are occupied by the substrate. When all active sites are occupied, the enzyme is saturated. At this saturation point, adding more substrate makes no difference to the reaction rate.