AP BIOLOGY

Detailed explanation-1: -The correct answer: The factor which does NOT affect enzyme activity is d. Free Energy.

Detailed explanation-2: -Enzyme concentration does not affect the enzyme activity directly as substrate concentration determine it until the presence of the substrate in an excess amount. Enzymes are the assemblage of protein subunits and hence function under specific temperature and pH range only.

Detailed explanation-3: -Several factors affect the rate at which enzymatic reactions proceed-temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.