AP BIOLOGY

BIOCHEMISTRY

POLAR AND NONPOLAR MOLECULES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Chromatography separates a mixture of dyes on the basis of their
A
density, weight and polarity
B
gravity
C
boiling point
Explanation: 

Detailed explanation-1: -Ion exchange chromatography separates the components of a mixture based on their charge, in addition to or instead of their size. In essence, positively (cations) or negatively (anions) charged ions are separated using different stationary phases and different pH mobile phases.

Detailed explanation-2: -Normal/reversed-phase chromatography separates molecules by their polarity. The stationary phase contains either highly polar (normal-phase) or highly non-polar (reverse-phase) functional groups that interact with analytes proportionate to their polarity.

Detailed explanation-3: -You probably used paper chromatography as one of the first things you ever did in chemistry to separate out mixtures of colored dyes-for example, the dyes which make up a particular ink.

There is 1 question to complete.