AP BIOLOGY

Detailed explanation-1: -Answer and Explanation: Methylene blue stains the nucleus, mitochondria and ribosomes of a cell. This is because methylene blue is capable of staining nucleic acids and they are most abundantly found in these three different organelles.

Detailed explanation-2: -The methylene blue stain makes nuclei more visible although it stains both nuclei and cytoplasm; the eosin mainly colors the cytoplasm and cell membranes.

Detailed explanation-3: -Methylene blue is a cationic dye which is also known as basic dyes. It has a positive ionic charge so it will interact with cell nuclei that have a negative ionic charge of the tissue (4). This dye can react with anionic groups as phosphate groups of nucleic acids (DNA and RNA) which is commonly used as nuclear stains.

Detailed explanation-4: -Methylene Blue is a cationic stain and it is used to stain the nucleus because it has a positive ionic charge, it will interact with tissue cell nuclei that have a negative ionic charge.

Detailed explanation-5: -What does methylene blue stain? Methylene blue, also known as methylthioninium chloride, can be used to stain or highlight parts of animal, bacteria, and blood tissue specimens in microbiology. Use it to closely observe the shapes, structures, and locations of dead cells and other tissues!