AP BIOLOGY

Detailed explanation-1: -MPF is a dimer consisting of cyclin B and the Cdc2 protein kinase. A variety of further studies have confirmed this role of cyclin B, as well as demonstrating the regulation of MPF by phosphorylation and dephosphorylation of Cdc2 (Figure 14.16). In mammalian cells, cyclin B synthesis begins in S phase.

Detailed explanation-2: -This suggested the key role of this molecule in the induction of the M-phase of cell cycle in all eukaryotic cells. After numerous efforts, MPF was identified as a complex of Cyclin-Dependent Kinase 1 (CDK1) and its regulatory subunit cyclin B [6–9].

Detailed explanation-3: -In other words, MPF is a system consisting of both cyclin B-Cdk1 that directs mitotic entry and Gwl that suppresses the anti-cyclin B-Cdk1 phosphatase, PP2A-B55.

Detailed explanation-4: -Maturation-promoting factor (abbreviated MPF, also called mitosis-promoting factor or M-Phase-promoting factor) is the cyclin-Cdk complex that was discovered first in frog eggs. It stimulates the mitotic and meiotic phases of the cell cycle.

Detailed explanation-5: -Summary. Cyclin-dependent kinases (CDKs) are protein kinases characterized by needing a separate subunit-a cyclin-that provides domains essential for enzymatic activity. CDKs play important roles in the control of cell division and modulate transcription in response to several extra-and intracellular cues.