AP BIOLOGY

Detailed explanation-1: -During metaphase, the kinetochore microtubules pull the sister chromatids back and forth until they align along the equator of the cell, called the equatorial plane. There is an important checkpoint in the middle of mitosis, called the metaphase checkpoint, during which the cell ensures that it is ready to divide.

Detailed explanation-2: -Metaphase. During metaphase, spindle fibers attach to the centromere of each pair of sister chromatids (see Figure below). The sister chromatids line up at the equator, or center, of the cell. This is also known as the metaphase plate.

Detailed explanation-3: -Metaphase follows prophase. During metaphase, the chromosomes align in the center of the cell at the equatorial plate and the spindle fibers attach to the centromeres of the chromosomes.