AP BIOLOGY

Detailed explanation-1: -Explanation: Dihydroxyacetone phosphate (DHAP) is converted to glyceradehyde-3-phosphate (G3P) by the enzyme triose phosphate isomerase.

Detailed explanation-2: -Triosephosphate isomerase (TIM) catalyzes the reaction to convert dihydroxyacetone phosphate into glyceraldehyde 3-phosphate, and vice versa. In most organisms, its functional oligomeric state is a homodimer; however, tetramer formation in hyperthermophiles is required for functional activity.

Detailed explanation-3: -The regulatory process involves a glycolytic enzyme, triosephosphate isomerase (Tpi1), and its product, glyceraldehyde-3-phosphate (GAP).

Detailed explanation-4: -Mechanism of Triose phosphate isomerase. A four-residue segment of the loop H-bonds with the phosphate group of the substrate. Without the loop, the enediol intermediate would eliminate phosphate, with the end products being inorganic phosphate and toxic methylglyoxal.