AP BIOLOGY

Detailed explanation-1: -In the fourth step in glycolysis the enzyme Fructose-bisphosphate aldolase cleaves 1, 6-bisphosphate into two three-carbon isomers: dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate.

Detailed explanation-2: -Aldolase Function This enzyme plays a pivotal role during the fourth step of glycolysis. In layman’s terms, it helps convert sugar into energy through all the cells in the body. Not only that, but it also plays a role in the reverse pathway; gluconeogenesis.

Detailed explanation-3: -Aldolase is involved in glycolysis, gluconeogenesis, and fructose metabolism. The catalytic mechanism of a class I aldolase utilizes a Schiff-base intermediate for the cleavage of Fru 1, 6-P2 and Fru 1-P [34]. Class II aldolase requires Zn2+ in the active site [35].

Detailed explanation-4: -Aldolases are essential enzymes in the glycolysis pathway and catalyze the reaction cleaving fructose/tagatose 1, 6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate.