AP BIOLOGY

Detailed explanation-1: -Which substrate is used in the last step of glycolysis? Explanation: The last step in glycolysis is the transfer of phosphoryl group from phosphoenolpyruvate to ADP catalyzed by pyruvate kinase.

Detailed explanation-2: -Which of the following substrates can be used in the last step of glycolysis? Ans. Here, the correct answer is option ‘b’ phosphoenolpyruvate. The last step in glycolysis is accompanied by the transfer of a phosphoryl group from phosphoenolpyruvate to ADP which is catalyzed by pyruvate kinase.

Detailed explanation-3: -Pyruvate kinase is an enzyme that catalyzes the conversion of phosphoenolpyruvate and ADP to pyruvate and ATP in glycolysis and plays a role in regulating cell metabolism.

Detailed explanation-4: -Pyruvate kinase is the enzyme involved in the last step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP.

Detailed explanation-5: -It is the last step of the EMP pathway or glycolysis. Pyruvate kinase transfers a phosphate group from PEP to ADP, forming ATP and pyruvate or pyruvic acid. 2 ATP molecules and 2 pyruvate molecules are produced. This is also a substrate-level phosphorylation.