AP BIOLOGY

Detailed explanation-1: -Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1).

Detailed explanation-2: -SCNT stand for somatic cell nuclear transfer. It is a laboratory technique for creating an ovum with a donor nucleus.

Detailed explanation-3: -Here we summarize recent advances in SCNT technology and its potential applications for both reproductive and therapeutic cloning. Keywords: somatic cell nuclear transfer, epigenetic reprogramming, therapeutic cloning, reproductive cloning, iPSC.

Detailed explanation-4: -somatic cell nuclear transfer (SCNT), technique in which the nucleus of a somatic (body) cell is transferred to the cytoplasm of an enucleated egg (an egg that has had its own nucleus removed).

Detailed explanation-5: -During SCNT, scientists remove the nucleus from a healthy egg cell. They then transplant a nucleus from another cell into the egg cell. The resulting embryo can be used to create a cloned organism (reproductive cloning), or to generate embryonic stem cells that genetically match the nucleus donor (therapeutic cloning).