AP BIOLOGY

HEREDITY

LINKED GENES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
A
DNA is negatively charged so attracted to the positive end of the unit
B
DNA is positively charged to attracted to the negative end of the unit
C
the agarose gel in negatively charged
D
the agarose gel is positively charged
Explanation: 

Detailed explanation-1: -DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

Detailed explanation-2: -Why do the fragments of DNA in gel electrophoresis move away from the negative cathode? DNA is negatively charged and attracted to the positive anode. DNA is positively charged and attracted to the positive anode.

Detailed explanation-3: -Because DNA and RNA are negatively charged molecules, they will be pulled toward the positively charged end of the gel.

Detailed explanation-4: -Why is the DNA sample to be separated by gel electrophoresis always loaded at the cathode or negative end of the power source? The gel acts as a molecular sieve: because nucleic acid molecules carry negative charges on their phosphate groups, they all travel toward the positive pole in an electric field.

Detailed explanation-5: -DNA consist of a phosphate backbone which is a negatively charged, hence when the DNA is placed in gei-electrophoresis it always moves towards anode, as the anode is positively charged.

There is 1 question to complete.