AP BIOLOGY

LABORATORY REVIEW

DIFFUSION AND OSMOSIS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The presence of glucose should be tested by using
A
iodine
B
Benedict’s Solution
C
Sudan
D
Biuret
Explanation: 

Detailed explanation-1: -We can use a special reagent called Benedict’s solution to test for simple carbohydrates like glucose. Benedict’s solution is blue but, if simple carbohydrates are present, it will change colour – green/yellow if the amount is low and red if it is high.

Detailed explanation-2: -Generally, Benedict’s test detects the presence of aldehydes, alpha-hydroxy-ketones, and hemiacetals, including those that occur in certain ketoses.

Detailed explanation-3: -The acidic conditions and heat break the glycosidic bond in sucrose through hydrolysis process. The products of hydrolysis process are reducing sugars (glucose and fructose) which can be detected by Benedict’s reagent.

Detailed explanation-4: -Benedict’s reagent can be used to test for glucose. The test involves heating a solution of the sugar to be tested with Benedict’s reagent and observing the colour change of blue to orange. Benedict’s reagent will give a positive test result for glucose but not for starch.

There is 1 question to complete.