AP BIOLOGY

Detailed explanation-1: -AO is a fluorescent dye that intercalates selectively into nucleic acids and has been used to detect RNA and DNA in brain tissues.

Detailed explanation-2: -Interactions of the DNA intercalator acridine orange, with itself, with caffeine, and with double stranded DNA.

Detailed explanation-3: -Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein.

Detailed explanation-4: -Propidium iodide is a DNA dye and an intercalating agent. It is in the same chemical family as ethidium bromide.

Detailed explanation-5: -Acridine Orange (AO) is a nucleic acid selective metachromatic stain useful for cell cycle determination. AO interacts with DNA and RNA by intercalation or electrostatic attraction respectively. DNA intercalated AO fluoresces green (525nm); RNA electrostatically bound AO fluoresces red (>630nm).