LABORATORY REVIEW
MOLECULAR BIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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sticky ends
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blunt ends
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Detailed explanation-1: -The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’-or 3’-strand, which are known as overhangs. These overhangs are most often generated by a staggered cut of restriction enzymes.
Detailed explanation-2: -Overhangs are often generated in molecular biology by use of DNA endonucleases. Larger overhangs of several nucleotides, such as those created by restriction endonucleases, are often called “sticky-ends” since a DNA molecule with complimentary sequence in the overhang region can anneal to each other.
Detailed explanation-3: -A ‘sticky’ end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases.
Detailed explanation-4: -noun. informal. an unpleasant finish or death (esp in the phrase come to or meet a sticky end)