LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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to join pieces of DNA from different sources
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to destroy DNA
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To cut the DNA using restriction enzymes
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Detailed explanation-1: -The sticky ends of the two fragments stick together by complementary base pairing. However, there are still gaps in the sugar-phosphate backbones of the DNA double helix at the junction sites where the gene and plasmid DNA meet. Once they are joined by ligase, the fragments become a single piece of unbroken DNA.
Detailed explanation-2: -Ligation can join together fragments of DNA from different sources. Ligation is often used for DNA cloning.
Detailed explanation-3: -Sticky ends can be joined by using DNA ligases. Each restriction enzyme functions by inspecting the length of a DNA sequence.
Detailed explanation-4: -When two strands of DNA are cut with the same restriction enzyme, they produce complementary single stranded overhangs called sticky ends. Sticky ends can be used to connect the two complementary strands of DNA together using the enzyme DNA ligase.
Detailed explanation-5: -A sticky-end fragment can be ligated not only to the fragment from which it was originally cleaved, but also to any other fragment with a compatible sticky end. Restriction enzymes having different recognition sites may have identical (compatible!) sticky overhangs.