LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Adding sugar to the mixture.
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Decreasing the pH from 7 to 3
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Increasing the temperature from 20
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Adding water to the mixture.
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Detailed explanation-1: -How can the activity of a human amylase enzyme be increased during a laboratory experiment? Decreasing the pH from 7 to 3.
Detailed explanation-2: -At lower temperatures, the enzyme salivary amylase is deactivated and at higher temperatures, the enzyme is denaturated. Therefore, more time will be taken by enzyme to digest the starch at lower and higher temperatures. At 37° C, the enzyme is most active, hence, takes less time to digest the starch.
Detailed explanation-3: -At low temperatures the amylase will break starch down slowly due to reduced kinetic energy. At high temperatures the amylase will break starch down slowly or not at all due to denaturation of the enzyme’s active site .
Detailed explanation-4: -It was observed that the amylase activity increases with rising in temperature but declines with further increase in temperature which was contrary to the expected results. The experiment was carried out in different temperature conditions to determine the influence of temperature on each reaction.