LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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5’ T T T A A A G 3’
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3’ T T T A A A G 5’
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5’ A A A T T T C 3’
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3’ U U U A A A G 5’
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Detailed explanation-1: -DNA is always synthesized in the 5’-to-3’ direction, meaning that nucleotides are added only to the 3’ end of the growing strand.
Detailed explanation-2: -Since DNA polymerase requires a free 3’ OH group for initiation of synthesis, it can synthesize in only one direction by extending the 3’ end of the preexisting nucleotide chain. Hence, DNA polymerase moves along the template strand in a 3’–5’ direction, and the daughter strand is formed in a 5’–3’ direction.
Detailed explanation-3: -For the DNA strand 5’-TACGATCATAT-3’ the correct complementary DNA strand is: 3’-ATGCTAGTATA-5"&#…
Detailed explanation-4: -Chunks of DNA, called Okazaki fragments, are then added to the lagging strand also in the 5’ to 3’ direction. This type of replication is called discontinuous as the Okazaki fragments will need to be joined up later.