LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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GUACGA
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GTACGA
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CATGCT
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CTACGT
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Detailed explanation-1: -During transcription, the DNA of a gene serves as a template for complementary base-pairing, and an enzyme called RNA polymerase II catalyzes the formation of a pre-mRNA molecule, which is then processed to form mature mRNA (Figure 1).
Detailed explanation-2: -The template strand serves in mRNA synthesis while the other strand is called coding strand as its base sequence is same as that of newly synthesized mRNA. So, the sequence of mRNA will be identical to the given sequence of coding strand except for the presence of uracil in place of thymine in mRNA.
Detailed explanation-3: -The DNA triplet is the template for which complementary mRNA are coded. Adenine is coded as uracil (because RNA does not have a thymine base), cytosine as guanine, guanine as cytosine, thymine as adenine. TAC is coded as AUG and CAG as GUC.