LABORATORY REVIEW
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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positive, negative, positive
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positive, positive, negative
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negative, positive, negative
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positive, negative, negative
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Detailed explanation-1: -In gel electrophoresis, the positive pole is called the anode and the negative pole is called the cathode; therefore, the charged particles will migrate to the respective nodes.
Detailed explanation-2: -As previously mentioned, gel electrophoresis involves an electrical field; in particular, this field is applied such that one end of the gel has a positive charge and the other end has a negative charge.
Detailed explanation-3: -DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
Detailed explanation-4: -DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.