AP BIOLOGY

LABORATORY REVIEW

MOLECULAR BIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Mode of DNA replication is
A
Conservative and bidirectional
B
Semiconservative and unidirectional
C
Semiconservative and bidirectional
D
Conservative and unidirectional
Explanation: 

Detailed explanation-1: -In Escherichia coli DNA replication is semi-conservative and bidirectional. In 1958, Matthew meselson and Stahl experimented on Escherichia coli. This experiment proved that in newly synthesized DNA one strand is parental and one is the new one.

Detailed explanation-2: -The mode of replication of DNA in E coli is semi conservative and bidirectional. In semiconservative two strands of daughter DNA form from single parent DNA. Bidirectional replication takes place in two directions. Like other bacteria, the replication occurs in its chromosomes.

Detailed explanation-3: -DNA replication is a bidirectional process as the strands of DNA are antiparallel, i.e., 3’-5’ in one strand and 5’-3’ in the other direction. The DNA polymerase synthesizes the DNA in 5’-3’ direction.

Detailed explanation-4: -Replication begins at a specific site in the DNA called the origin of replication (oriC). DNA replication is bidirectional from the origin of replication.

Detailed explanation-5: -DNA replication is semiconservative, meaning that each strand in the DNA double helix acts as a template for the synthesis of a new, complementary strand. This process takes us from one starting molecule to two “daughter” molecules, with each newly formed double helix containing one new and one old strand.

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